Molekularbiologie

Studienzentrum Labor Krone

Publikationen

HIV Pre-exposure prophylaxis during the SARS-CoV-2 pandemic: Results from a prospective observational study in Germany
Mona Uhrmacher, Adriane Skaletz-Rorowski, Sandeep Nambiar, Axel Jeremias Schmidt, Patrick Ahaus, Katja Serova, Isabel Mordhorst, Arne Kayser, Janet Wach, Carsten Tiemann, Dieter Münstermann, Norbert H. Brockmeyer and Anja Verena Potthoff
Front Pub Health (2022, submitted)

Effective high-throughput RT-qPCR screening for SARS-CoV-2 infections in children
Felix Dewald, Isabelle Suárez, Ronja Johnen, Jan Grossbach, Roberto Moran-Tovar, Gertrud Steger, Alexander Joachim, Gibran Horemheb Rubio, Mira Fries, Florian Behr, Joao Kley, Andreas Lingnau, Alina Kretschmer, Carina Gude, Guadelupe Beazes-Flores, David Laveaga del Valle, Alberto Roblero-Hernandez, Jesus Magana-Cerino, Adriana Torres Hernandez, Jesus Ruiz-Quinones, Konstantin Schega, Viktoria Linne, Lena Junker, Marie Wunsch, Eva Heger, Elena Knops, Veronica Di Cristanziano, Meike Meyer, Christoph Hünseler, Lutz T. Weber, Jan-Christoffer Lüers, Gustav Quade, Hilmar Wisplinghoff, Carsten Tiemann, Rainer Zotz, Hassan Jomaa, Arthur Pranada, Ileana Herzum, Paul Cullen, Franz-Josef Schmitz, Paul Philipsen, Georg Kirchner, Cornelius Knabbe, Martin Hellmich, Michael Buess, Anna Wolf, Annelene Kossow, Johannes Niessen, Sebastian Jeworutzki, Jörg-Peter Schräpler, Michael Lässig, Jörg Dötsch, Gerd Fätkenheuer, Rolf Kaiser, Andreas Beyer, Jan Rybniker, Florian Klein
Nature Comm. (2022, accepted)

Prevalence of SARS-CoV-2 in Pregnant Women Assessed by RT-PCR in Franconia, Germany: First Results of the SCENARIO Study (SARS-CoV-2 prEvalence in pregNAncy and at biRth In FrancOnia)
Alexander Hein, Sven Kehl, Lothar Häberle, Carsten Tiemann, Rebecca Peuker, Denise Mereutanu, Florian M Stumpfe, Florian Faschingbauer, Kirstin Meyer-Schlinkmann, Martin C Koch, Franz Kainer, Ulf Dammer, Hanna Philipp, Carolin Kladt, Michael G Schrauder, Stefan Weingärtler, Volker Hanf, Arndt Hartmann, Matthias Rübner, Holm Schneider, Jos Lelieveld, Matthias W Beckmann, Lena A Wurmthaler, Peter A Fasching, Michael O Schneider
Geburtshilfe Frauenheilkd. 2022 Feb 11;82(2):226-234. doi: 10.1055/a-1727-9672. eCollection 2022 Feb

HIV pre-exposure prophylaxis was associated with no impact on sexually transmitted infection prevalence in a high-prevalence population of predominantly men who have sex with men, Germany, 2018 to 2019
Hendrik Streeck, Klaus Jansen, Trevor A Crowell, Allahna Esber, Heiko K Jessen, Christiane Cordes, Stefan Scholten , Stephan Schneeweiss , Norbert Brockmeyer, Christoph D Spinner, Markus Bickel, Stefan Esser, Jukka Hartikainen, Albrecht Stoehr, Clara Lehmann, Ulrich Marcus, Jörg Janne Vehreschild, Alexandra Knorr, Anna-Lena Brillen, Carsten Tiemann, Merlin L Robb, Nelson L Michael
Euro Surveill. 2022 Apr;27(14):2100591. doi: 10.2807/1560-7917.ES.2022.27.14.2100591.

Influence of dietary supplementation of short-chain fatty acid sodium propionate in people living with HIV (PLHIV)
V Brauckmann, S Nambiar, A Potthoff, S Höxtermann, J Wach, A Kayser, C Tiemann, A K Schuppe, N H Brockmeyer, A Skaletz-Rorowski J Eur Acad Dermatol Venereol. 2022 Feb 17. doi: 10.1111/jdv.18006

Decreasing prevalence and stagnating incidence of Hepatitis C-co-infection among a cohort of HIV-1-positive patients, with a majority of men who have sex with men, in Germany, 1996-2019
Amrei Krings, Daniel Schmidt, Karolin Meixenberger, Norbert Bannert, Dieter Münstermann, Carsten Tiemann, Christian Kollan, Barbara Gunsenheimer-Bartmeyer
J Viral Hepat. 2022 Mar 18. doi: 10.1111/jvh.13670.

Recommendations for accurate genotyping of SARS-CoV-2 using amplicon-based sequencing of clinical samples
Slawomir Kubik, Ana Claudia Marques, Xiaobin Xing, Janine Silvery, Claire Bertelli, Flavio De Maio, Spyros Pournaras, Tom Burr, Yannis Duffourd, Helena Siemens, Chakib Alloui, Lin Song, Yvan Wenger, Alexandra Saitta, Morgane Macheret, Ewan W Smith, Philippe Menu, Marion Brayer, Lars M Steinmetz, Ali Si-Mohammed, Josiane Chuisseu, Richard Stevens, Pantelis Constantoulakis, Michela Sali, Gilbert Greub, Carsten Tiemann, Vicent Pelechano, Adrian Willig, Zhenyu Xu
Clin Microbiol Infect . 2021 Jul;27(7):1036.e1-1036.e8. doi: 10.1016/j.cmi.2021.03.029. Epub 2021 Apr 2.

Mouthrinses against SARS-CoV-2: anti-inflammatory effectivity and a clinical pilot study
Matthias Schürmannn, Mohamed Aljubeh, Carsten Tiemann, Holger Sudhoff
Eur Arch Otorhinolaryngol. 2021 Dec;278(12):5059-5067. doi: 10.1007/s00405-021-06873-8. Epub 2021 May 22.

Factors Associated with Testing for HIV and Hepatitis C among Behaviorally Vulnerable Men in Germany : a cross-sectional analysis upon enrollment into an observational cohort
Crowell TA, Qian H, Tiemann C, Lehmann C, Boesecke C, Stoehr A, Kartikainen J, Esser S, Bickel M, Spinner CD, Schneeweiß S, Cordes C, Brockmeyer N, Jessen H, Robb ML, Michael NL, Jansen K, Streeck H for the BRAHMS Study Team.
AIDS Res Ther. (2021) Aug 16;18(1):52. doi: 10.1186/s12981-021-00378-4.

Sexual behavior and prevention of sexually transmitted infections taking the SARS-CoV-2 pandemic into account. Data from a sexual healthcare and medicine center-WIR
Norbert H Brockmeyer, Anja Potthoff, Wiltrud Knebel-Brockmeyer, Britta Köhler, Sandeep Nambiar, Janet Wach, Tobias Rodrigues Martins, Mona Uhrmacher, Ann-Kathrin Schuppe, Carsten Tiemann 6, Andre Kasper, Miriam Basilowski, Arne Kayser, Adriane Skaletz-Rorowski
Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. 2021 Nov;64(11):1440-1451. doi: 10.1007/s00103-021-03441-7. Epub 2021 Oct 19.

Wie lässt sich die Eliminierung von Hepatitis B, C und D in Deutschland messen? Ergebnisse eines interdisziplinären Arbeitstreffens. 
Zimmermann, R., Külper-Schiek, W., Steffen, G. et al.  Bundesgesundheitsbl (2020).

STI in times of PrEP: high prevalence of chlamydia, gonorrhea, and mycoplasma at different anatomic sites in men who have sex with men in Germany
Klaus Jansen, Gyde Steffen, Anja Potthoff, Ann-Kathrin Schuppe, Daniel Beer, Heiko Jessen, Stefan Scholten, Petra Spornraft-Ragaller, Viviane Bremer, Carsten Tiemann BMC Infect Dis 20, 110 (2020).

Developmental validation of the monSTR identity panel, a forensic STR multiplex assay for massively parallel sequencing
Silvery J, Ganschow S, Wiegand P, Tiemnn C Forensic Sci Int Genet , 46 (2020)
doi: 10.1016/j.fsigen.2020.102236. Epub 2020 Jan 14

Evaluation of a laboratory-developed test for simultaneous detection of norovirus and rotavirus by real-time RT-PCR on the Panther Fusion® system
Robert K Kulis-Horn , Carsten Tiemann Eur J Clin Microbiol Infect Dis. 2020 Jan;39(1):103-112. doi: 10.1007/s10096-019-03697-7. Epub 2019 Sep 10.

HIV in der Schwangerschaft – eine Übersicht
Tiemann C
Die Hebamme 2019; 32(01): 46-53, DOI: 10.1055/a-0830-7310

Development of a multiplex forensic identity panel for massively parallel sequencing and its systematic optimization using design of experiments
Ganschow S, Silvery J, Tiemann C
Forensic Science International: Genetics 39, 32-43 (2019)

toaSTR: A web application for forensic STR genotyping by massively parallel sequencing
Ganschow S, Silvery J, Kalinowski J, Tiemann C
Forensic Science International: Genetics 37, 21-28 (2018)

Multicenter Evaluation of Two Next-Generation HIV-1 Quantitation Assays, Aptima Quant Dx and Cobas 6800, in Comparison to the RealTi m e HIV-1 Reference Assay
Frank Wiesmann, Robert Ehret, Gudrun Naeth, Martin Däumer, Jörg Fuhrmann, Rolf Kaiser, Christian Noah, Martin Obermeier, Gunnar Schalasta, Carsten Tiemann, Eva Wolf, Heribert Knechten, Patrick Braun J Clin Microbiol . 2018 Sep 25;56(10):e00292-18. doi: 10.1128/JCM.00292-18. Print 2018 Oct.

Not again! Effect of previous test results, age group and reason for testing on (re-)infection with Chlamydia trachomatis in Germany
Alexandra Sarah Lang, Matthias An der Heiden, Klaus Jansen, Andrea Sailer, Viviane Bremer, Sandra Dudareva, Chlamydia trachomatis laboratory sentinel team
BMC Infect Dis. 2018 Aug 25;18(1):424. doi: 10.1186/s12879-018-3323-2.

toaSTR: A web-based forensic tool for the analysis of short tandem repeats in massively parallel sequencing data
Sebastian Ganschow, Peter Wiegand, Carsten Tiemann Forensic Science International: Genetics Supplement Series, 2017

Establishment of a voluntary electronic Chlamydia trachomatis laboratory surveillance system in Germany, 2008 to 2014
S Dudareva-Vizule, K Haar, A Sailer, K Jansen, O Hamouda, H Wisplinghoff, C Tiemann, E Pape , V Bremer, Chlamydia trachomatis laboratory sentinel team
Eurosurveillance, Volume 22, Issue 6, 09 February 2017

Prevalence of Chlamydia trachomatis infection in women, heterosexual men and MSM visiting HIV counselling institutions in North Rhine-Westphalia, Germany – should Chlamydia testing be scaled up? Anne Lallemand, Viviane Bremer, Klaus Jansen, Stine Nielsen, Dieter Münstermann, Andreas Lucht, Carsten Tiemann, 2016, BMC Infectious Diseases 16:610

Local Health Study: Aufsuchende medizinische Angebote für Sexarbeiterinnen in Bochum. R.-E. Klingenberg, S. Mannherz, Prof. Dr. N. H. Brockmeyer, J. Wach, R. Winter, C. Tiemann, A. Lucht, S. Schaffer, M. Eickel, A. Skaletz-Rorowski, 2016,
DOI 10.1007/s00105-016-3882-3

Go Local Health Study: Aufsuchende medizinische Angebote bei Sexarbeiterinnen in Bochum. Rozalia-Eva Scheitz, Sonja Mannher, Janet Wach, Ralf Winter, Mechthild Eickel, Carsten Tiemann, Norbert H. Brockmeyer, 2015 JDDG

Draft genome sequence of Bacillus anthracis UR-1 isolated from a
German heroin user.
Christian Rückert, Katharina Licht, Joern Kalinowski, Christophe Espirito Santo, Markus Antwerpen, Matthias Hanczaruk, Udo Reischl, Thomas Holzmann, André Gessner, Carsten Tiemann and Gregor Grass 2012, Journal of Bacteriology

Draft genome sequence of Bacillus anthracis BF-1 isolated from Bavarian cattle
Markus Antwerpen, Diogo Proença, Christian Rueckert, Katharina Licht, Joern Kalinowski, Matthias Hanczaruk, Carsten Tiemann and Gregor Grass 2012, Journal of Bacteriology

H1N1, der Erreger des Jahres 2009 – Pandemie ohne Panik
Tiemann C, 2009, Trillium Report

A Cryptographic Biometric Authentication System based on Genetic Fingerprints.
Ulrike Korte, Michael Krawczak, Johannes Merkle, Rainer Plaga, Matthias Niesing, Carsten Tiemann, Han Vinck, Ulrich Martini:. Sicherheit 2008: 263-276

Frequency of very low HCV viremia detected by a highly sensitive HCV-RNA assay.
Fytili P, Tiemann C, Wang C, Schulz S, Schaffer S, Manns MP, Wedemeyer H.
J Clin Virol. 2007 Aug;39(4):308-11. Epub 2007 Jul 12.

Multicenter evaluation of the new Abbott RealTime assays for quantitative detection of human immunodeficiency virus type 1 and hepatitis C virus RNA.
Schutten M, Peters D, Back NK, Beld M, Beuselinck K, Foulongne V, Geretti AM, Pandiani L, Tiemann C, Niesters HG.
J Clin Microbiol. 2007 Jun;45(6):1712-7. Epub 2007 Apr 4.

Detection of Treponema pallidum in the vitreous by PCR.
Müller M, Ewert I, Hansmann F, Tiemann C, Hagedorn HJ, Solbach W, Roider J, Nölle B, Laqua H, Hoerauf H.
Br J Ophthalmol. 2007 May;91(5):592-5. Epub 2006 Nov 15.

Ultra-rapid detection of Chlamydia trachomatis by real-time PCR in the LightCycler using SYBR green technology or 5′-nuclease probes.
Eickhoff M, Laue T, Ruckes T, Cramer SO, Krupp G, Tiemann C.
Clin Lab. 2003;49(5-6):217-25.

Rapid DNA typing of HLA-B27 allele by real-time PCR using lightcycler technology.
Tiemann C, Vogel A, Dufaux B, Zimmer M, Krone JR, Hagedorn HJ.
Clin Lab. 2001;47(3-4):131-4.

Leitfaden Molekulare Diagnostik
Kap. 5, Die Detektion von PCR Produkten, C.Tiemann
in: Thiemann, Cullen, Klein (2006) – Verlag Wiley-VCH

Molekulare Diagnostik (2. Auflage)
Kap. 4, Die Detektion von PCR Produkten, C.Tiemann
in: Thiemann, Cullen, Klein (2015) – Verlag Wiley-VCH

Multi-target PCRs – the new multi-tool of STI diagnostics. Kulis-Horn, Robert, Muenstermann, Dieter,Tiemann, Carsten. (2019). P029 Sexually Transmitted Infections. 95. A93.1-A93. 10.1136/sextrans-2019-sti.237.

Automation and optimization strategies in a molecular lab result in an improved workflow for STI surveillance studies. Tiemann, Carsten, Steffen, Gyde, Schuppe, Ann-Kathrin, Bremer, Viviane, Jansen, Klaus. (2019). Sexually Transmitted Infections. 95. A91.3-A91. 10.1136/sextrans-2019-sti.234.

Development and validation of the monSTR forensic identity panel – a multiplex STR Kit for MPS
Silvery J., Ganschow S., Siemens H., Wiegand P., Tieman C.
28th Congress of the International Society for Forensic Genetics, Prag (2019)

Influence of HIV-status and prep use on high STI prevalences in MSM in germany, 2018. Jansen, Klaus, Steffen, Gyde, Schuppe, Ann-Kathrin, Bremer, Viviane, Tiemann, Carsten. (2019). O08. Sexually Transmitted Infections. 95. A56.1-A56. 10.1136/sextrans-2019-sti.149.

Detection of mycoplasma genitalium macrolide resistance using the open channel of the panther fusion® system. Kulis-Horn, Robert & Jansen, Klaus & Jensen, Jorgen & Tiemann, Carsten. (2019). P607 Sexually Transmitted Infections. 95. A269.1-A269. 10.1136/sextrans-2019-sti.675.

Design of Experiments: Wie aus monSTR ein High-Performance Identity Panel wurde
Ganschow S, Tiemann C
39. Spurenworkshop, Jena (2019)

Massively Parallel Sequencing für die hochauflösende STR-Typisierung – Validation des 21-plex Kits monSTR
Silvery J, Tiemann C
14. Jahrestagung der Deutschen Gesellschaft für Abstammungsbegutachtung (DGAB), Freiburg (2018)

High positivity of sexually transmitted infections in men who have sex with men in the times of HIV pre-exposure prophylaxis (PrEP) – results of a cross-sectional multicentre study in Germany, 2018
Steffen G, Jansen K, Ziesenis AK, Münstermann D, Tiemann C. European Scientific Conference on Applied Infectious Disease Epidemiology, Malta (2018)

toaSTR: a user-friendly tool for STR allele calling in MPS data
Ganschow S, Tiemann C
ENFSI DNA Working Group Meeting, Rom (2018)

toaSTR: die Web-App für eine einfache und effiziente STR-Genotypisierung mit MPS
Ganschow S, Tiemann C
38. Spurenworkshop, Basel (2018)

Der Sequenz auf der Spur: MPS-Konkordanzstudie zum GEDNAP-Ringversuch
Silvery J, Tiemann C
38. Spurenworkshop, Basel (2018)

toastr.de – the Swiss knife for next-generation STR genotyping
Ganschow S, Tiemann C
18th European Forensic DNA Working Group Meeting, Innsbruck (2017)

NGS in forensics – from complex to simple
Tiemann C
18th European Forensic DNA Working Group Meeting, Innsbruck (2017)

MULTICENTRIC PRECISION ANALYSIS OF TWO FULLY-AUTOMATED HIV VIRAL LOAD ASSAYS, APTIMA HIV-1 QUANT DX AND ROCHE HIV-1 FORUSE ON THE COBAS 6800, IN COMPARISON TO ABBOTT REALTIME HIV-1
F. Wiesmann , P. Braun, B. Haase, G. Naeth, G. Schalasta , M. Obermeier , C. Tiemann , B. Thiele , R. Kaiser , E. Wolf , C. Noah , H. Knechten, 20th ESCV, Stresa (IT) 2017

Application of a multiplex PCR assay for detection of sexually transmitted infections
Robert Kasimir Kulis-Horn and Carsten Tiemann, 10th EMMD, Noordwijk, 2017

Automatisierungslösungen im PCR-Labor
C. Tiemann, Seegene Symposium , Kassel 2017

STI Diagnostik I/II
R. Kulis-Horn, Seegene Symposium, Kassel 2017

Multiplex-PCRs (Gastro, Respi, STI) in der klinischen Anwendung
C. Tiemann, 6. REMMDI, Regensburg 2017

Implant-associated infection diagnosis using sonication and high-throughput 16S rRNA gene sequencing: a standardization and validation approach.
Ganschow S, Tiemann C ECCMID (2016)

Prevalence of Mycoplasma genitalium in patients visiting HIV counselling institutions in North-Rhine-Westphalia, Germany (STI-HIT study). Lallemand A, Bremer V, Jansen K, Nielsen S, Münstermann D, Lucht A, Tiemann C. ISSTDR (2015)

High prevalence of genital infections with Mycoplasma genitalium in female sex workers reached at their working place in Germany: the STI-Outreach study. Jansen K, Bremer V, Steffen G, Sarma N, Nielsen S., Münstermann D, Lucht A, Tiemann C; ISSTDR (2015)

High prevalence of genital infections with Chlamydia, gonorrhoea and trichomonas in female sex workers reached at their working place in Germany: the STI-Outreach study. Jansen K. (1), Bremer V. (1), Steffen G. (1), Sarma N. (1), Nielsen S. (1), Münstermann D. (2), Lucht A. (2), Tiemann C. ECCMID (2015)

Prävalenz genitaler Infektionen mit Chlamydia trachomatis (CT), Neisseria gonorrhoea (NG) und Trichomonas vaginalis (TV) in Klienten der HIV-Testberatungsstellen in Nordrhein-Westfalen: die STI-HIT Studie Bremer V, Jansen K, Steffen G, Sarma N, Nielsen S, Lucht A, Münstermann D, Tiemann C, DGSTI (2014)

Hohe Prävalenz genitaler Infektionen mit Chlamydien, Gonorrhö und Trichomonaden bei am Arbeitsplatz erreichten Sexarbeiterinnen in Deutschland: die STI-Outreach-Studie. Jansen K, Bremer V, Steffen G, Sarma N, Nielsen S, Münstermann D, Lucht A, Tiemann C, DGSTI 2014

Prevalence of genital infections with Chlamydia trachomatis, Neisseria gonorrhea and Trichomonas vaginalis in hard-to-reach sex workers in Germany: The STI-Outreach Study. Klaus Jansen, Viviane Bremer, Gyde Steffen, Navina Sarma, Dieter Münstermann, Andreas Lucht, Carsten Tiemann , DÖÄK (2013)

Prevalence of genital infections with Chlamydia trachomatis, Neisseria gonorrhoea and Trichomonas vaginalis in clients of public HIV counseling institutions in North Rhine-Westphalia, Germany: the STI-HIT study. Viviane Bremer, Klaus Jansen, Gyde Steffen, Navina Sarma, Stine Nielsen, Andreas Lucht, Dieter Münstermann, Carsten Tiemann, DÖAK (2013)

Comparison of PCR and electrospray-ionization mass spectrometry (ESI-MS) with
species-specific diagnostic algorithms during a biodefense external quality

assurance exercise.
  Bryan R. Thoma, Daniela Knoop, Andrea Hartmann, Carsten Tiemann, Lothar Zöller, Christin Hinz, Daniela Jacob, Roland Grunow and Sabine Schmoldt, DGHM Jahrestagung 2012

Evaluation of analytical sensitivity and specificity of the biothreat assay for clinical Bacillus anthracis diagnostics by the PLEX-ID™ System. M. Hanczaruk, B. Thoma, M. Antwerpen, S. Schmoldt, C. Tiemann,
D. Knoop, A. Hartmann, L. Zöller, G. Grass, VAAM Jahrestagung 2012

PlexID System: evaluation of analytical sensitivity and specificity of the biothreat assay for clinical Bacillus anthracis diagnostics. B.R. Thoma, M. Hanczaruk, M.H. Antwerpen, S. Schmold, D. Knoop, A. Hartmann, C. Tiemann , G. Grass.  Medical Biodefence Conference 2012

Identification of orthopoxviruses by PCR/electrospray-ionization mass spectrometry. H. Meyer, L. Dobrzykowski, D. Knoop, A. Hartmann, B.R. Thoma, C. Tiemann , S. Schmold,  Medical Biodefence Conference 2012

Rapid identification of infectious pathogens with PCR/electrospray-ionization TOF mass spectrometry. C. Tiemann , D. Knoop, A. Hartmann, C. Busse, European Meeting on Molecular Diagnostics, 2011

Improvement of a RNA isolation method for carotid endarterectomy samples embedded in cryo-medium. D. Knoop, C. Lanckohr, G. Theilmeier, C. Tiemann, European Meeting on Molecular Diagnostics 2009

Evaluation of the KingFisher Flex© for high throughput extraction of DNA and RNA from clinical samples. A. Hartmann, D.Knoop, T.Zinn, C.Tiemann, European Meeting on Molecular Diagnostics 2009

Evaluation of testing cadaveric serum for HIV, HCV and HBV viraemia.
C Tiemann and D. Knoop, European Meeting on Molecular Diagnostics 2007

Performance Evaluation of a New Instrument for Genomic orViral DNA or RNA Extraction for HBV Viral Load Testing. C. Tiemann , D. Knoop , C. Dunn , S. Schaffer, European Meeting on Molecular Diagnostics 2007

Rapid Detection of Norovirus Genogroups By Automated RNA-Extraction and Real-time RT-PCR
C. Tiemann, A. Vogel, B. Dufaux, M. Zimmer, D. Münstermann,J.-R. Krone and H.-J. Hagedorn, European Meeting on Molecular Diagnostics 2005

Rapid and sensitive detection of PCR products in clinical diagnostics using the electrophoresis system Caliper 1000. C. Tiemann, A. Vogel, B. Dufaux, M. Zimmer, J.-R. Krone and H.-J. Hagedorn, European Meeting on Molecular Diagnostics 2003

Rapid Detection of Chlamydia trachomatis by Real-time PCR Using SYBR-Green I
C. Tiemann, A. Vogel, B. Dufaux, M. Zimmer, J.-R. Krone and H.-J. Hagedorn, European Meeting on Molecular Diagnostics 2001

STI on the rise – lessons learned from high risk population screening
Tiemann C, 13th International Symposium on Molecular Diagnostics Graz (2020)

Multiplex-PCR in der STI-Diagnostik
Tiemann C, DGHM-Tagung Göttingen (2019)

The BRAHMS study – a prospective, multicenter study to assess point prevalence, incidence and recurrence of sexually transmitted infections in men who have sex with men.
Streeck, Jansen, Crowell, Jessen, Cordes, Scholten, Schneeweiss, Brockmeyer, Spinner, Bickel, Esser, Hartikainen, Stoehr, Lehmann, Brillen, Marcus, Vehrenschild, Tiemann, Robb, Michael.
DÖAK 2019 – Deutsch-Österreichischer AIDS-Kongress

Multi-target PCR – the multi-tool in molecular diagnostics?
Tiemann C, 12th International Symposium on Molecular Diagnostics Graz (2018)

Multi-Target-PCRs – neue Multi-Tools der STI-Diagnostik
Tiemann C, DSTIG-Kongress Bochum (2018)

NGS in forensics – from complex to simple
Tiemann C, 18th European Forensic DNA Working Group Meeting, Innsbruck (2017)

Praktische Erfahrungen mit dem PantherFUSION System
C. Tiemann, Hologic Anwender-Treffen 2017

Multiplex-PCRs (Gastro, Respi, STI) in der klinischen Anwendung
Tiemann C, Regensburger Meeting für angewandte molekulare Diagnostik (REMMDI) 2017

Application of multiplex real-time PCR assays for STI patients
C. Tiemann, ECCMID, Wien 2017

Next Generation Sequencing für die forensische Genetik. Entwicklung und Evaluation eines High-Fidelity 10-plex STR-Assays
M. Sc. Sebastian Ganschow, Tiemann C DGAB Tagung 2017, Bielefeld

Implant-associated Infection Diagnosis using Sonication and Molecular Methods
M. Sc. Sebastian Ganschow, Tiemann C 11th CeBiTec Symposium, Bielefeld University, 2016

Prävalenz genitaler Infektionen in verschiedenen Risiko-Populationen.
C. Tiemann, Hologic Anwender-Treffen 2015

Laborvergleich für Aptima Combo 2® External Quality Assessment for HOLOGIC Panther® and Tigris® System  Nucleic Acid Detection for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG)
C. Tiemann, Hologic Anwender-Treffen 2015

Genitale Infektionen mit Chlamydien, Gonorrhö und Trichomonas bei „hard-to-reach“ Sexarbeiterinnen in Deutschland: Ergebnisse der STI-Outreach-Studie
Klaus Jansen, Viviane Bremer, Stine Nielsen, Gyde Steffen, Navina Sarma Carsten Tiemann, Dieter Münstermann, Andreas Lucht, Sven Schaffer, NRW-LIGA-Meeting (2014)

Möglichkeiten und Grenzen automatisierter Nachweissysteme in der molekularen Diagnostik.
C. Tiemann, STI-Jubiläumskongress der DSTIG, Berlin, 2012

PlexID – zukünftige Möglichkeiten zur Erweiterung der Routinediagnostik beim Nachweis von Infektionserregern.
C. Tiemann, 10. Wissenschaftliches Abbott Symposium, 2012

Identification of orthopoxviruses by PCR/electrospray-ionization mass spectrometry.
H. Meyer, L. Dobrzykowski, D. Knoop, A. Hartmann, B.R. Thoma, C. Tiemann , S. Schmold,
Medical Biodefence Conference 2012

Rapid identification of infectious pathogens with PCR/electrospray-ionization TOF mass spectrometry.
C. Tiemann , D. Knoop, A. Hartmann, C. Busse
European Meeting on Molecular Diagnostics, 2011

Implementierung der PCR/ESI-TOF MS Technologie im Routinelabor
C. Tiemann, DGHM Tagung 2011

Rapid identification of mixed- and single bacterial and fungal infections with PCR coupled to electrospray-ionization TOF mass spectrometry.
C. Tiemann, D. Knoop, A. Hartmann, DGHM Tagung 2011

Molekulare Identifizierung von Pathogenen mittels PCR / ESI-TOF Massenspektrometrie
C. Tiemann, Regensburger Meeting für angewandte Molekulare Diagnostik (REMMDI), 2011

Identifizierung und Charakterisierung von Bakterien und Viren mittels PlexID – Evaluierungsbericht.
C.Tiemann, 7. Abbott Anwender-Forum Molekulare Diagnostik, 2011

Massenspektometrische Untersuchung von STR-Systemen mittels ESI-TOF-MS/PCR – am Beispiel des aktuellen GEDNAP-Ringversuches C. Tiemann, 31. Spurenworkshop der Deutschen Gesellschaft für Rechtsmedizin, 2011

RealTime High Risk HPV Diagnostik : erste Praxiserfahrung
C. Tiemann,  5. Abbott Anwender-Forum, 2009

Abbott Molecular Automation Advisory Board (Co-Chair)
C. Tiemann, Amsterdam-Meeting 2010

„Neue Grippe“ – H1N1
C. Tiemann, Rotary Club Herford, 2009

Abbott RealTime High Risk HPV vs HC“ (Digene)
C. Tiemann, Fortbildungstagung Klinische Zytologie, München, 2009

Chlamydien-Screening: erster Erfahrungsbericht
C. Tiemann,  4. Abbott Anwender-Forum, 2008

Gen-Profile und Identifizierungswahn – Möglichkeiten und moralische Grenzen
C. Tiemann, FoeBuD 2008

Immunologie / Infektionskrankheiten

Studienzentrum Labor Krone

Publikationen

Guttmann, S., Bunte, S., Eschrig, S., Janzen, N., Klemens, J., Koenemann, Y., … Meyer-Schlinkmann, K. M. (2023). Dried blood spot eluates are suitable for testing of SARS-CoV-2 IgG antibodies targeting Spike protein 1 and Nucleocapsid protein. Biochemistry and Biophysics Reports, 34, 101479. https://doi.org/https://doi.org/10.1016/j.bbrep.2023.101479

Guttmann S, Fazio J, Gunkel T et al.: Comparative performance measurement of agglutination assays for antibody detection against Treponema pallidum, 74. Jahrestagung DGHM 2022.

Hagedorn HJ, Meyer-Schlinkmann K, Fazio J, Guttmann S, Münstermann D: Screening in the context of syphilis diagnostics: threshold ranges for automated Syphilis immunoassays, 74. Jahrestagung DGHM 2022.

Hein, A., Kehl, S., Häberle, L., Tiemann, C., Peuker, R., Mereutanu, D., … Schneider, M. O. (2022). Prevalence of SARS-CoV-2 in Pregnant Women Assessed by RT-PCR in Franconia, Germany: First Results of the SCENARIO Study (SARS-CoV-2 prEvalence in pregNAncy and at biRth In FrancOnia) TT – Prävalenz von SARS-CoV-2 bei schwangeren Frauen ermittelt durch RT. GebFra – DGGG-Gesellschaftsausgaben, (01), 226–234.

Lange, B., Jaeger, V. K., Harries, M., Rücker, V., Streeck, H., Blaschke, S., … Karch, A. (2023). Estimates of protection against SARS-CoV-2 infection and severe COVID-19 in Germany before the 2022/2023 winter season – the IMMUNEBRIDGE project. MedRxiv, 2023.02.16.23285816. https://doi.org/10.1101/2023.02.16.23285816

Meyer-Schlinkmann, K.M. & Kulis-Horn, R. (2022). Pooling-Verfahren zum Nachweis von SARS-CoV-2 in Humanproben. Forum Sanitas – Das Informative Medizinmagazin, 02, 45-47.

Schulze-Wundling, K., Ottensmeyer, P. F., Meyer-Schlinkmann, K. M., Deckena, M., Krüger, S., Schlinkert, S., … Streeck, H. (2023). Immunity Against SARS-CoV-2 in the German Population. Deutsches Arzteblatt International, 120(19), 337–344. https://doi.org/10.3238/arztebl.m2023.0072